Classically, the 3 untranslated region (3 UTR) is that region in eukaryotic protein-coding genes from the translation termination codon to the polyA signal. It is transcribed as an integral part of the mRNA encoded by the gene.
However, there exists another kind of RNA, which consists of the 3 UTR alone, without all other elements in mRNA such as 5 UTR and coding region. The importance of independent 3 UTR RNA (referred as I3 UTR) was prompted by results of artificially introducing such RNA species into malignant mammalian cells.
Since 1991, we found that the middle part of the 3 UTR of the human nuclear factor for interleukin-6 (NF-IL6) or C/EBP gene exerted tumor suppression effect in vivo.
Our subsequent studies showed that transfection of C/EBP 3 UTR led to down-regulation of several genes favorable for malignancy and to up-regulation of some genes favorable for phenotypic reversion Technical engineering reports are divided into manageable sections to aid comprehension of the material. In this video, Dr Andrew Garrard takes us through the .
Also, it was shown that the sequences near the termini of the C/EBP 3 UTR were important for its tumor suppression activity. Then, the C/EBP 3 UTR was found to directly inhibit the phosphorylation activity of protein kinase CPKC in SMMC-7721, a hepatocarcinoma cell line.
Recently, an AU-rich region in the C/EBP 3 UTR was found also to be responsible for its tumor suppression.
Recently we have also found evidence that the independent C/EBP 3 UTR RNA is actually exists in human tissues, such as fetal liver and heart, pregnant uterus, senescent fibroblasts etc 26 Jun 2013 - People arguably spend as much time analyzing test failures as they do writing tests. This necessitates good functional test reports. A good .
Through 1990 s to 2000 s, world scientists found several 3 UTR RNAs that functioned as artificial independent RNAs in cancer cells and resulted in tumor suppression.
Interestingly, majority of genes for these RNAs have promoter-like structures in their 3 UTR regions, although the existence of their transcribed products as independent 3 UTR RNAs is still to be confirmed Human Anatomy High Impact List of Articles PPts Journals 806. Case Report: Anatomy & Physiology: Current Research, 2017: 273. DOI: 10.4172/2161- .
Our studies indicate that the independent 3 UTR RNA is a novel non-coding RNA species whose function should be the regulation not of the expression of their original mRNA, but of some essential life activities of the cell as a whole.